NGS各种组学建库原理（图解）

"pythonic生物人"的第61篇分享。

本文分享NGS中各种组学建库原理图【llumina官网】，分三个方向：Seq—DNA（DNA层面）、Seq—RNA（RNA层面）、Seq—Single-Cell（单细胞层面）。

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本文将了解到什么？

1、DNA层面各组学建库原理图解：Seq—DNA
DNA variants
Low levels of DNA
Epigenetics
DNA–protein interactions
Protein–protein interactions

2、RNA层面各组学建库原理图解：Seq—RNA
RNA transcription
RNA–protein interactions
Low levels of RNA
RNA modifications
RNA structure

3、单细胞层面各组学建库原理图解：Seq—Single-Cell
Low-level RNA detection
Low-level DNA detection
Integrated DNA and RNA analysis

正文开始啦

1、DNA层面：Seq—DNA

具体分如下几个方向

• DNA variants
• Low levels of DNA

• Epigenetics

CAB-Seq

• DNA–protein interactions

ChIP-Seq

ATAC-SeqHi-C 、3-C

• Protein–protein interactions

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2、RNA层面：Seq—RNA

分以下几类：

• RNA transcription

• RNA–protein interactions

• Low levels of RNA

UMI Method

• RNA modifications

• RNA structure

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3、单细胞层面：Seq—Single-Cell

可分以下几个方面：

• Low-level RNA detection

• Low-level DNA detection

• Integrated DNA and RNA analysis

https://www.illumina.com/

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